Isadora Marcante was born in Porto Alegre, Rio Grande do Sul, Brazil. Her parents are the personal trainers Cintia Szeckir and Enio Luiz Marcante. She started acting at the age of 19. She studied Acting for Film at School of Actors and graduated from Pontifical Catholic University of Rio Grande do Sul with a Bachelor of Arts in Journalism. Her first role was in the short film Memories (2013).