Jesi Mullins was born and raised in Crookston, MN to parents Richard and Lynnette Mullins.
After being recruited from a high school performance as Catherine in "Pippin" Jesi earned her Bachelor of Fine Arts in theatrical performance degree at the University of North Dakota. During her collegiate studies, she also traveled to Italy to study physical theatre at Accademia dell'Arte in Arezzo, Italy as well as the acclaimed singing workshop The Tuscany Project.
In New York, Jesi studied with the famed HB Studio under the tutelage of Lorraine Serabian.