Carlie Cain was born on June 21, 1989 in Wilkesboro, North Carolina. She lived and completed school in her hometown. She graduated from North Wilkes High School in 2007 and attended Wilkes Community College for two years. She soon transferred to Appalachian State University in Boone, North Carolina where she received her Bachelor of Arts in History. During her school tenure, she worked with the comedy troupe, Short Bus TV and other acting projects.