None observed, though there was hint / mention of animal procreating, we were not even shown scenes of this.
Violence & Gore
Unrated
A person was seen to use a tool to hit an animal (tool was not shown to be actually touching animal).
An animal bit a person for its own protection, but the bitten person was not severely hurt. A bit of blood was seen on where the person was hurt, but appears to be minor external skin scraping.
Profanity
Unrated
The most severe is the Japanese term for "fool".
Alcohol, Drugs & Smoking
Unrated
None observed to be using / consuming, though there was a scene where a store worker was helping to transport crates of drinks (maybe beer), but not clearly discernible.
Frightening & Intense Scenes
Unrated
The reality of what the animal center was doing may instill distress and sadness to very young children.