In a comical tone, the singing owls keep saying to the audience how Rango would die throughout the entire movie.
A small fly drinks from a spilled "cactus juice" puddle at the bar. It immediately flips over dead, and one of it's eyes pop out of it's head. Brief, but could be disturbing.
There is a character who has an arrow through his eye and exiting the back of his head, the character is seen by Rango who jokes about it.
In a comedic way, the singing owls sing and play instruments hanging from the ceiling with a noose around their necks singing about how the robbers are going to be hung
The character of Rattlesnake Jake may be scary to some young children. He does have a Gatling gun at the tip of his tail where the rattle would be and threatens to kill (and to enjoy killing) the sympathetic characters.
Rattlesnake Jake coils around the Mayor and drags him off, screaming. He also strangles Beans into unconsciousness while threatening her death. May be unsettling.
Although some of things mentioned above seem harsh for a family film, the film overall is innocent, the scenes that are harsh often contain a comic element or some distraction to prevent the film from becoming overly dark.
It's a little more grown-up than your average animated film (mainly Pixar films) due to a few frightening characters, thematic material, some stronger language ("hell" and "damn" in the vocabulary) and "uglier" character design.