When Lincoln lay dying at the Petersen House, he is naked under the covers.

There is one scene showing John Wilkes Booth making love to his girlfriend Lucy Hale. However, this only lasts a few moments and no frontal nudity other than the chest and stomach is seen. Booth's girlfriend is wearing a nightgown.

As obviated by the title, Abraham Lincoln is shown being shot in the head by John Wilkes Booth. After shooting Lincoln, he stabs Major Rathbone, Lincoln's guest at the theater, in the arm. It is later shown bleeding profusely. Lincoln's blood is also shown when Dr. Leale feels for the wound and at a scene near the end when someone changes Lincoln's pillow, which is covered in his blood. Also, at the beginning of the movie, John Wilkes Booth getting shot in the back of the neck by Boston Corbett is foreseen, and at the end of the movie, he is shot in the back of the neck by Corbett and dragged over to a porch, where blood is seen oozing out.

Near the middle of the film, when Booth is asked how he and the conspirators could get to Lincoln and kill him, he says "Maybe in his office, he lets in any d--n fool... ." Lincoln also says the "d word" when arguing with Secretary of War Edwin Stanton during a meeting. At the end of the film, someone yells Corbett, the soldier who shot Booth, and says, "We were supposed to take him alive, you son of a b--ch!" However, no real strong language is used in the movie.

Near the beginning of the movie, Booth and his fellow conspirators are shown passing around a bottle of some alcohol. Also around the same time, people are seen celebrating the Union victory and drinking. There are also several scenes in a bar.

Also, General and future president Ulysses S. Grant is seen smoking a cigar at a meeting.

The assassination scene may frighten some young children, as well as that of Booth being shot.