Penis Parker ejaculates silly string on 2 different characters during the film. One character is implied to be masturbating while sitting in a lawn chair.

The hobo implies that he has taught freshmen students about anal.

The hobo and Peter are caught in a porta-potty together for Spiderman's "Superhero Colonoscopy"

Nothing graphic, just comic book style fighting, mainly for comedy effect.

There are guns.

One use of faggot, a few fucks and shits. One character's first name is Penis. Another character's name is Professional Fapster (masturbation reference)

The hobo is constantly swearing at and to Penis Parker

Two characters are stoners and smoke weed out of a sunscreen bottle but it is brief and does not look realistic.

One song in the credits references getting high

Suicide attempt at the start of the film. An explosion in one scene is louder than the rest of the film. Not too scary. The ending may make some people sad.